Iphepha lamva nje elipapashwe kwiJenali yePhysiology yenze nzulu imeko yeziphumo zokukhuthaza ulutsha lokuzilolonga kwizinto ezigugayo, ukwakhiwa komsebenzi wangaphambili owenziwe ngeempuku zelebhu ezisondela esiphelweni sobomi bazo bendalo obunokufikelela kwivili lokuzilolonga elinobunzima.
Iphepha elineenkcukacha ezininzi, "Isiginitsha yemolekyuli echaza ukuziqhelanisa nokuguga kunye ne-vivo partial reprogramming in skeletal muscle," idwelisa ababhali abadibeneyo be-16, abathandathu kubo badibene ne-U of A. Umbhali ohambelanayo nguKevin Murach, unjingalwazi oncedisayo kwi-U yeSebe lezeMpilo le-A, iNtsebenzo yoLuntu kunye noLonwabo, kunye nombhali wokuqala nguRonald G. Jones III, Ph.D.umfundi waseMurach's Molecular Muscle Mass Regulation Laboratory.
Kweli phepha, abaphandi bathelekisa iigundane eziguga ezikwazi ukufikelela kwivili lokuzilolonga elinobunzima kunye neempuku eziye zafumana i-epigenetic reprogramming ngokuchazwa kwezinto ze-Yamanaka.
Imiba ye-Yamanaka yimiba emine yoshicilelo lweprotheyini (echongwe njengo-Oct3/4, i-Sox2, i-Klf4 kunye ne-c-Myc, ehlala ishunqulelwa kwi-OKSM) enokubuyisela iiseli ezichazwe kakhulu (ezifana neseli yesikhumba) emva kwe-stem cell, eyi-stem cell. ubume obuncinci kunye nokulungelelaniswa ngakumbi.I-Nobel Prize in Physiology or Medicine yanikezelwa kuGqr. Shinya Yamanaka ngokufunyanwa ngo-2012. Kwiidosi ezichanekileyo, ukunyanzeliswa kwezinto ze-Yamanaka kuwo wonke umzimba kwiimpuku kunokuphucula iimpawu zokuguga ngokulinganisa ukuguquguquka okuqhelekileyo kulutsha oluninzi. iiseli.
Kwimiba emine, iMyc ibangelwa kukusebenzisa izihlunu zamathambo.I-Myc inokusebenza njengendalo evuselela i-reprogramming stimulus kwi-muscle, iyenza ibe yindawo eluncedo yokuthelekisa phakathi kweeseli eziye zahlelwa ngokutsha ngokubonakaliswa kwezinto ze-Yamanaka kunye neeseli eziye zahlengahlengiswa ngokuzilolonga - "reprogramming" kwimeko yokugqibela ebonisa indlela ukuvuselela kokusingqongileyo kunokuguqula ukufikeleleka kunye nokubonakaliswa kwemfuza.
Abaphandi bathelekisa i-skeletal muscle yeempuku eziye zavunyelwa ukuba zisebenzise emva kwexesha ebomini kwi-skeletal muscle yeempuku ezigqithise kakhulu i-OKSM kwimisipha yazo, kunye neempuku eziguqulwe ngofuzo ezilinganiselwe kwi-overexpression ye-Myc nje kwimisipha yazo.
Ekugqibeleni, iqela linqume ukuba umthambo ukhuthaza iprofayili ye-molecular ehambelana ne-epigenetic partial programming.Oko kukuthi: ukuzivocavoca kunokulinganisa iinkalo zeprofayili ye-molecular of muscles eziye zavezwa kwizinto ze-Yamanaka (ngaloo ndlela ibonisa iimpawu ze-molecular zeeseli zolutsha ngakumbi).Lo mphumela unenzuzo wokuzilolonga unokuthi ngokuyinxenye kubangelwe kwizenzo ezithile zeMyc kwisihlunu.
Nangona bekuya kuba lula ukuqikelela ukuba ngenye imini sinokukwazi ukuxhaphaza i-Myc kwisisipha ukuze sifezekise imiphumo yokuzilolonga, ngaloo ndlela sisigcina umsebenzi onzima, uMurach uyalumkisa ukuba kuya kuba sisigqibo esingalunganga sokwenza.
Okokuqala, i-Myc ayinakuze ikwazi ukuphindaphinda yonke imiphumo esezantsi yokuzivocavoca umzimba wonke.Ikwangunobangela wamathumba kunye nomhlaza, ngoko ke kukho iingozi zendalo zokuyilawula intetho yayo.Endaweni yoko, uMurach ucinga ukuba ukusebenzisa iMyc kungasetyenziswa ngcono njengeqhinga lokulinga ukuqonda indlela yokubuyisela ukuziqhelanisa nokuziqhelanisa nezihlunu ezindala ezibonisa ukusabela okuhlayo.Kusenokwenzeka ukuba ikwayindlela yokuhlawula ngaphezulu kwempendulo yoosomajukujuku kubunzima obuthi zero okanye abantu abavaleleke ekuphumleni ebhedini abanomthamo oqingqiweyo wokuzilolonga.I-Myc ineziphumo ezininzi, ezilungileyo nezimbi, ngoko ke ukuchaza ezona ziluncedo kunokukhokelela kunyango olukhuselekileyo olunokusebenza ebantwini phantsi kwendlela.
UMurach ubona uphando lwabo njengoqinisekiso olongezelelweyo lokuzilolonga njengepolypill."Ukuzivocavoca sesona siyobisi sinamandla," utshilo, kwaye kufuneka kuthathelwe ingqalelo njengokuphucula impilo - kunye nokwandisa ubomi - unyango kunye namayeza kunye nokutya okunempilo.
UMurach kunye noJones ababhali abasebenzisanayo kwi-U of A baquka unjingalwazi wenzululwazi wokuzilolonga uNicholas Greene, kunye nabaphandi abanegalelo uFrancielly Morena Da Silva, uSeongkyun Lim noSabin Khadgi.
Ixesha lokuposa: Mar-02-2023